jednocząsteczkowego FRET do badania kinetyki   zmian konformacyjnych białek

Połączenie szybkiego mieszania mikroprzepływowego i trójkolorowego jednocząsteczkowego FRET do badania kinetyki   zmian konformacyjnych białek

Jednocząsteczkowy rezonansowy switch energii Förstera (FRET) doskonale nadaje się do badania kinetyki zmian konformacyjnych białek ze względu na wysoką czułość i zdolność do rozpoznawania poszczególnych subpopulacji w układach heterogenicznych. Jednak najpowszechniejsze podejście wykorzystujące dwa fluorofory może monitorować tylko jedną odległość na raz, a użycie trzech fluoroforów do jednoczesnego monitorowania wielu odległości zostało w dużej mierze ograniczone do fluktuacji równowagi. Tutaj pokazujemy, że trójkolorowy jednocząsteczkowy FRET można łączyć z szybkim mieszaniem mikroprzepływowym w celu zbadania zmian konformacyjnych w białku od milisekund do minut. W połączeniu z mieszaniem ręcznym wydłużyliśmy kinetykę do 1 godziny, co odpowiada łącznemu zakresowi 5 rzędów wielkości w czasie.

Zbadaliśmy konwersję monomeru w protomer tworzącej pory toksyny, cytolizyny A (ClyA), jedną z największych znanych przemian konformacyjnych białka. Specyficzne dla miejsca znakowanie ClyA trzema fluoroforami umożliwiło nam jednoczesne śledzenie kinetyki trzech odległości wewnątrzcząsteczkowych i ujawniło wcześniej niewykryty związek pośredni. Połączenie trójkolorowego jednocząsteczkowego FRET z szybkim mieszaniem mikroprzepływowym zapewnia zatem podejście do badania mechanizmów złożonych procesów biomolekularnych z wysoką rozdzielczością czasową.

 

Identyfikacja 7 000-9 000  białek  z linii komórkowych i tkanek za pomocą mikroprzepływu pojedynczego strzału LC-MS/MS

Obecnym trendem w proteomice jest akwizycja danych w „pojedynczym ujęciu” za pomocą LC-MS/MS, ponieważ upraszcza to przepływy pracy i obiecuje lepszą przepustowość i dokładność ilościową niż schematy obejmujące rozległe frakcjonowanie próbek. Jednak podejścia jednokrotne mogą ucierpieć z powodu ograniczonego pokrycia proteomu, gdy są wykonywane przez akwizycję zależną od danych (ssDDA) w systemach nanoprzepływowych LC. W przypadku zastosowań, w których ilości próbek nie są ograniczone, badanie to pokazuje, że wysokie pokrycie proteomem można uzyskać przy użyciu systemu mikroprzepływowego LC-MS/MS obsługującego kolumnę o średnicy wewnętrznej 1 mm × 150 mm, przy natężeniu przepływu 50 μl/min i sprzężonych do spektrometru masowego Orbitrap HF-X.

Wyniki wskazują na identyfikację ~9000 białek z 50 μg strawionego białka z   korzeni Arabidopsis , 7500 z mysiej grasicy i 7300 z ludzkich komórek raka piersi w ciągu three godzin analizy w jednym przebiegu. Dynamiczny zakres oznaczania ilościowego białek mierzony metodą iBAQ obejmował 5 rzędów wielkości, a analiza powtórzeń wykazała, że ​​mediana współczynnika zmienności wynosiła poniżej 20%. Podsumowując , badanie to pokazuje, że ssDDA metodą μLC-MS/MS jest solidną metodą kompleksowej i wielkoskalowej analizy proteomu, którą w przyszłości można rozszerzyć o szybszą chromatografię i metody pozyskiwania danych niezależnych od danych.̀.

WDR62 cDNA Clone
MBS1278452-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 330

WDR62 cDNA Clone
MBS1278452-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1430

WDR41 cDNA Clone
MBS1278785-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 350

WDR41 cDNA Clone
MBS1278785-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1525

WDR16 cDNA Clone
MBS1266694-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 330

WDR16 cDNA Clone
MBS1266694-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1430

WDR66 cDNA Clone
MBS1266972-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 1245

WDR66 cDNA Clone
MBS1266972-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 5560

WDR54 cDNA Clone
MBS1268926-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 175

WDR54 cDNA Clone
MBS1268926-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 735

WDR31 cDNA Clone
MBS1269060-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR31 cDNA Clone
MBS1269060-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR82 cDNA Clone
MBS1269111-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR82 cDNA Clone
MBS1269111-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR86 cDNA Clone
MBS1269205-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 275

WDR86 cDNA Clone
MBS1269205-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1190

WDR88 cDNA Clone
MBS1268193-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR88 cDNA Clone
MBS1268193-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR47 cDNA Clone
MBS1268397-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 520

WDR47 cDNA Clone
MBS1268397-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 2295

WDR59 cDNA Clone
MBS1267248-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 330

WDR59 cDNA Clone
MBS1267248-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1430

WDR77 cDNA Clone
MBS1267348-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR77 cDNA Clone
MBS1267348-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR76 cDNA Clone
MBS1267434-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR76 cDNA Clone
MBS1267434-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR44 cDNA Clone
MBS1267496-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 600

WDR44 cDNA Clone
MBS1267496-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 2655

WDR66 cDNA Clone
MBS1273393-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 880

WDR66 cDNA Clone
MBS1273393-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 3910

WDR47 cDNA Clone
MBS1273453-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 520

WDR47 cDNA Clone
MBS1273453-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 2295

WDR54 cDNA Clone
MBS1273667-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR54 cDNA Clone
MBS1273667-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR24 cDNA Clone
MBS1273900-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 745

WDR24 cDNA Clone
MBS1273900-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 3300

WDR46 cDNA Clone
MBS1274239-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 590

WDR46 cDNA Clone
MBS1274239-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 2605

WDR41 cDNA Clone
MBS1274442-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR41 cDNA Clone
MBS1274442-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR91 cDNA Clone
MBS1274578-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR91 cDNA Clone
MBS1274578-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR34 cDNA Clone
MBS1274709-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR34 cDNA Clone
MBS1274709-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR85 cDNA Clone
MBS1274748-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR85 cDNA Clone
MBS1274748-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR13 cDNA Clone
MBS1275052-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR13 cDNA Clone
MBS1275052-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR33 cDNA Clone
MBS1269354-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR33 cDNA Clone
MBS1269354-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR92 cDNA Clone
MBS1269465-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR92 cDNA Clone
MBS1269465-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR25 cDNA Clone
MBS1269628-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR25 cDNA Clone
MBS1269628-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR34 cDNA Clone
MBS1269761-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 425

WDR34 cDNA Clone
MBS1269761-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1860

WDR38 cDNA Clone
MBS1269862-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR38 cDNA Clone
MBS1269862-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR27 cDNA Clone
MBS1269863-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 435

WDR27 cDNA Clone
MBS1269863-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1910

WDR49 cDNA Clone
MBS1270547-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 435

WDR49 cDNA Clone
MBS1270547-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1910

WDR18 cDNA Clone
MBS1270831-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR18 cDNA Clone
MBS1270831-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR20 cDNA Clone
MBS1271465-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 330

WDR20 cDNA Clone
MBS1271465-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1430

WDR73 cDNA Clone
MBS1277186-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 385

WDR73 cDNA Clone
MBS1277186-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1690

WDR76 cDNA Clone
MBS1277238-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 330

WDR76 cDNA Clone
MBS1277238-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1430

WDR19 cDNA Clone
MBS1277262-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR19 cDNA Clone
MBS1277262-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR85 cDNA Clone
MBS1277295-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR85 cDNA Clone
MBS1277295-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR67 cDNA Clone
MBS1277315-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR67 cDNA Clone
MBS1277315-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR75 cDNA Clone
MBS1277561-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 780

WDR75 cDNA Clone
MBS1277561-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 3455

WDR20 cDNA Clone
MBS1277815-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR20 cDNA Clone
MBS1277815-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR60 cDNA Clone
MBS1272194-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 1155

WDR60 cDNA Clone
MBS1272194-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 5150

WDR65 cDNA Clone
MBS1275851-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 435

WDR65 cDNA Clone
MBS1275851-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1910

WDR53 cDNA Clone
MBS1276140-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR53 cDNA Clone
MBS1276140-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR90 cDNA Clone
MBS1276163-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 315

WDR90 cDNA Clone
MBS1276163-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1385

WDR63 cDNA Clone
MBS1271597-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 520

WDR63 cDNA Clone
MBS1271597-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 2295

WDR37 cDNA Clone
MBS1271703-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR37 cDNA Clone
MBS1271703-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR74 cDNA Clone
MBS1271879-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR74 cDNA Clone
MBS1271879-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR18 cDNA Clone
MBS1271880-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 435

WDR18 cDNA Clone
MBS1271880-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1910

Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514)
MR204616	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514)
MR204616L3	Origene Technologies GmbH	10 µg	Ask for price

WDR45L cDNA Clone
MBS1268101-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR45L cDNA Clone
MBS1268101-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR6 cDNA Clone
MBS1268803-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR6 cDNA Clone
MBS1268803-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR6 cDNA Clone
MBS1267661-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 1210

WDR6 cDNA Clone
MBS1267661-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 5405

WDR1 cDNA Clone
MBS1267204-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 330

WDR1 cDNA Clone
MBS1267204-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1430

WDR1 cDNA Clone
MBS1273887-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 330

WDR1 cDNA Clone
MBS1273887-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 1430

WDR7 cDNA Clone
MBS1274012-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 765

WDR7 cDNA Clone
MBS1274012-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 3400

WDR5 cDNA Clone
MBS1275747-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR5 cDNA Clone
MBS1275747-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR4 cDNA Clone
MBS1269790-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR4 cDNA Clone
MBS1269790-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

WDR3 cDNA Clone
MBS1272968-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 160

WDR3 cDNA Clone
MBS1272968-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 670

WDR4 cDNA Clone
MBS1276184-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 200

WDR4 cDNA Clone
MBS1276184-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 855

Lenti ORF particles, Wdr78 (GFP-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514), 200ul, >10^7 TU/mL
MR204616L4V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514), 200ul, >10^7 TU/mL
MR204616L3V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF clone of Wdr78 (mGFP-tagged) - Mouse WD repeat domain 78 (Wdr78)
MR209396L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (Wdr78)
MR209396L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr78 (mGFP-tagged ORF) - Rat WD repeat domain 78 (Wdr78), (10 ug)
RR208219L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr78 (Myc-DDK-tagged ORF) - Rat WD repeat domain 78 (Wdr78), (10 ug)
RR208219L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti-ORF clone of WDR78 (mGFP-tagged)-Human WD repeat domain 78 (WDR78), transcript variant 2
RC221613L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr48 (mGFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)
MR209851L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr73 (mGFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)
MR203438L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr62 (mGFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)
MR211422L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr62 (mGFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)
MR211595L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr42a (mGFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)
MR204846L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr20a (mGFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
MR208616L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)
MR209851L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)
MR203438L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)
MR211422L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)
MR211595L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti-ORF clone of WDR78 (Myc-DDK-tagged)-Human WD repeat domain 78 (WDR78), transcript variant 2
RC221613L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr42a (Myc-DDK-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)
MR204846L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
MR208616L3	Origene Technologies GmbH	10 µg	Ask for price

WDR3 (untagged)-Human cDNA clone MGC:61946 IMAGE:6745281, complete cds
SC126422	Origene Technologies GmbH	10 µg	Ask for price

Wdr73 (GFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)
MG203438	Origene Technologies GmbH	10 µg	Ask for price

Wdr36 (GFP-tagged) - Mouse WD repeat domain 36 (cDNA clone MGC:103340 IMAGE:5068941)
MG211095	Origene Technologies GmbH	10 µg	Ask for price

Wdr47 (GFP-tagged) - Mouse WD repeat domain 47 (cDNA clone MGC:8037 IMAGE:3586908)
MG211187	Origene Technologies GmbH	10 µg	Ask for price

Wdr59 (GFP-tagged) - Mouse WD repeat domain 59 (cDNA clone MGC:91308 IMAGE:30355918)
MG211357	Origene Technologies GmbH	10 µg	Ask for price

Wdr67 (GFP-tagged) - Mouse WD repeat domain 67 (cDNA clone MGC:60990 IMAGE:30016266)
MG211408	Origene Technologies GmbH	10 µg	Ask for price

Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)
MG211422	Origene Technologies GmbH	10 µg	Ask for price

Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)
MG211595	Origene Technologies GmbH	10 µg	Ask for price

Wdr48 (GFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)
MG209851	Origene Technologies GmbH	10 µg	Ask for price

Wdr32 (GFP-tagged) - Mouse WD repeat domain 32 (cDNA clone MGC:11993 IMAGE:3601889)
MG206473	Origene Technologies GmbH	10 µg	Ask for price

Wdr77 (untagged) - Mouse WD repeat domain 77 (cDNA clone MGC:6890 IMAGE:2654151), (10ug)
MC200277	Origene Technologies GmbH	10 µg	Ask for price

Wdr24 (untagged) - Mouse WD repeat domain 24 (cDNA clone MGC:47001 IMAGE:5148210), (10ug)
MC206644	Origene Technologies GmbH	10 µg	Ask for price

Wdr68 (untagged) - Mouse WD repeat domain 68 (cDNA clone MGC:58053 IMAGE:6409531), (10ug)
MC206690	Origene Technologies GmbH	10 µg	Ask for price

Wdr37 (untagged) - Mouse WD repeat domain 37 (cDNA clone MGC:58091 IMAGE:6817089), (10ug)
MC206152	Origene Technologies GmbH	10 µg	Ask for price

Wdr48 (untagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), (10ug)
MC217839	Origene Technologies GmbH	10 µg	Ask for price

Wdr73 (untagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), (10ug)
MC218435	Origene Technologies GmbH	10 µg	Ask for price

Wdr62 (untagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), (10ug)
MC223031	Origene Technologies GmbH	10 µg	Ask for price

Wdr62 (untagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), (10ug)
MC223128	Origene Technologies GmbH	10 µg	Ask for price

Wdr42a (GFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)
MG204846	Origene Technologies GmbH	10 µg	Ask for price

Wdr20a (GFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
MG208616	Origene Technologies GmbH	10 µg	Ask for price

WDR78 cloning plasmid
CSB-CL719620HU1-10ug	Cusabio	10ug	EUR 640.8
Description: A cloning plasmid for the WDR78 gene.

WDR78 cloning plasmid
CSB-CL719620HU2-10ug	Cusabio	10ug	EUR 986.4
Description: A cloning plasmid for the WDR78 gene.

Wdr42a (untagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38511 IMAGE:5352941), (10ug)
MC206626	Origene Technologies GmbH	10 µg	Ask for price

Wdr40b (untagged) - Mouse WD repeat domain 40B (cDNA clone MGC:76797 IMAGE:30431243), (10ug)
MC206734	Origene Technologies GmbH	10 µg	Ask for price

Wdr20a (untagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717), (10ug)
MC217866	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr4 (mGFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
MR206503L4	Origene Technologies GmbH	10 µg	Ask for price

Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)
MR209851	Origene Technologies GmbH	10 µg	Ask for price

Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)
MR203438	Origene Technologies GmbH	10 µg	Ask for price

Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)
MR211422	Origene Technologies GmbH	10 µg	Ask for price

Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)
MR211595	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
MR206503L3	Origene Technologies GmbH	10 µg	Ask for price

Wdr42a (Myc-DDK-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)
MR204846	Origene Technologies GmbH	10 µg	Ask for price

Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
MR208616	Origene Technologies GmbH	10 µg	Ask for price

WDR37 (untagged)-Human WD repeat domain 37 (cDNA clone MGC:26330 IMAGE:4794184), complete cds
SC123593	Origene Technologies GmbH	10 µg	Ask for price

Wdr4 (GFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
MG206503	Origene Technologies GmbH	10 µg	Ask for price

Wdr8 (untagged) - Mouse WD repeat domain 8 (cDNA clone MGC:28791 IMAGE:4501325), (10ug)
MC218347	Origene Technologies GmbH	10 µg	Ask for price

WDR59 (untagged)-Human hypothetical protein FLJ12270 (cDNA clone MGC:11230 IMAGE:3938206), complete cds
SC122523	Origene Technologies GmbH	10 µg	Ask for price

WDR77 (untagged)-Human methylosome protein 50, mRNA (cDNA clone MGC:4346 IMAGE:2822173), complete cds
SC126139	Origene Technologies GmbH	10 µg	Ask for price

Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
MR206503	Origene Technologies GmbH	10 µg	Ask for price

WDR78
CSB-CL719620HU1	Cusabio	10 μg plasmid + 200μl Glycerol	Ask for price

WDR78
CSB-CL719620HU2	Cusabio	10 μg plasmid + 200μl Glycerol	Ask for price

Wdr78/ Rat Wdr78 ELISA Kit
ELI-16965r	Lifescience Market	96 Tests	EUR 1063.2

WDR25 (untagged)-Human pre-mRNA splicing factor-like (cDNA clone MGC:4645 IMAGE:3529568), complete cds
SC122376	Origene Technologies GmbH	10 µg	Ask for price

WDR85 (untagged)-Human chromosome 9 open reading frame 112, mRNA (cDNA clone MGC:3209 IMAGE:3502107), complete cds
SC126100	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF particles, Wdr48 (GFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), 200ul, >10^7 TU/mL
MR209851L4V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr73 (GFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), 200ul, >10^7 TU/mL
MR203438L4V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), 200ul, >10^7 TU/mL
MR211422L4V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), 200ul, >10^7 TU/mL
MR211595L4V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr42a (GFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701), 200ul, >10^7 TU/mL
MR204846L4V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr20a (GFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717), 200ul, >10^7 TU/mL
MR208616L4V	Origene Technologies GmbH	200 µl	Ask for price

WDR78 siRNA
20-abx939775	Abbexa	Ask for price Ask for price	15 nmol 30 nmol

WDR78 siRNA
20-abx939776	Abbexa	Ask for price Ask for price	15 nmol 30 nmol

Lenti ORF particles, Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), 200ul, >10^7 TU/mL
MR209851L3V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), 200ul, >10^7 TU/mL
MR203438L3V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), 200ul, >10^7 TU/mL
MR211422L3V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), 200ul, >10^7 TU/mL
MR211595L3V	Origene Technologies GmbH	200 µl	Ask for price

WDR78 Antibody
E306990	EnoGene	100ug/200ul	EUR 275
Description: Available in various conjugation types.

WDR78 Antibody
MBS8527115-01mg	MyBiosource	0.1mg	EUR 345

Immunoterapia raka z użyciem NC410, białka LAIR-2 Fc   blokującego interakcję ludzkiego LAIR-kolagen 

Kolageny są głównym składnikiem macierzy zewnątrzkomórkowej i są funkcjonalnymi ligandami dla hamującego receptora immunologicznego związanego z leukocytami receptora podobnego do immunoglobuliny (LAIR)-1. LAIR-2 to wydzielane białko, które może działać jako receptor wabikowy, wiążąc kolagen z wyższym powinowactwem niż LAIR-1. Proponujemy, że kolageny promują unikanie odporności poprzez interakcję z LAIR-1 eksprymowanym na komórkach odpornościowych i że LAIR-2 uwalnia supresję immunologiczną za pośrednictwem LAIR-1.

Analiza publicznych zbiorów danych dotyczących ludzi pokazuje, że kolageny, LAIR-1 i LAIR-2 mają unikalne i nakładające się związki z przeżyciem w niektórych nowotworach. Zaprojektowaliśmy dimeryczny LAIR-2 z funkcjonalnym ogonem IgG1 Fc, NC410, i wykazaliśmy, że NC410 zwiększa ekspansję ludzkich komórek T i funkcję efektorową  in vivo  w mysim modelu choroby ksenogenicznego przeszczep przeciwko gospodarzowi. W humanizowanych mysich modelach nowotworów NC410 zmniejsza wzrost nowotworu zależny od komórek T. Analiza immunohistochemiczna ludzkich guzów wykazała, że ​​NC410 wiąże się z obszarami bogatymi w kolagen, w których  zlokalizowane są komórki odpornościowe LAIR-1 ⁺ . Nasze odkrycia pokazują, że NC410 może być nową strategią immunoterapii nowotworów w przypadku guzów z wykluczeniem immunologicznym.

Identyfikacja immunologiczna i charakterystyka białka  szkieletowego kapsydu  kodowanego przez UL26.5 wirusa Herpes Simplex typu 2

Wirus opryszczki pospolitej typu 2 (HSV2), patogen powodujący zmiany opryszczkowe narządów płciowych, ingeruje w układ odpornościowy gospodarza  poprzez  różne znane i nieznane mechanizmy. Wirus ten był używany do badania składu antygenowego wirusa. Surowicę rekonwalescencyjną od pacjentów zakażonych HSV2 zastosowano do identyfikacji antygenów wirusowych  za pomocą  elektroforezy białek 2-D i immunoblottingu. Surowica rozpoznawała głównie kilka białek szkieletowych kapsydu kodowanych przez gen UL26.5, głównie ICP35 .

Doniesiono, że białko to działa przejściowo w tworzeniu wirusów, ale nie ulega ekspresji w dojrzałych cząsteczkach wirusa. Dalsze badania immunologiczne sugerowały, że to białko wywołuje u myszy odpowiedzi swoistych przeciwciał i cytotoksycznych limfocytów T (CTL), ale te odpowiedzi nie dają klinicznego efektu ochronnego w odpowiedzi na prowokację HSV2 . Dane sugerują, że immunodominacja ICP35 może być wykorzystana do zaprojektowania zintegrowanego antygenu z innymi glikoproteinami wirusowymi.

Autoimmunologiczne sygnatury starzenia się i chorób neurodegeneracyjnych związanych z wiekiem są powiązane z funkcją mózgu i białkami rybosomalnymi

Biologiczne starzenie się to złożony proces charakteryzujący się upośledzeniem funkcji komórek i tkanek, w tym układu odpornościowego. W konsekwencji starzenie się wpływa na ekspresję i rozwój autoprzeciwciał oraz autoreaktywnych limfocytów T, co w ich sumie można uznać za autoimmunom osobnika. W tym badaniu przeanalizowaliśmy, czy zestawy cech autoimmunologicznych są powiązane z określonymi fenotypami, które tworzą sygnatury autoimmunologiczne związane z wiekiem i chorobami neurodegeneracyjnymi . Dane profilu autoprzeciwciał zdrowych osobników i pacjentów z bazy danych GEO wykorzystano do zbadania autoimmunologicznych sygnatur starzenia i trzech chorób neurodegeneracyjnych, w tym choroby Parkinsona (PD), choroby Alzheimera (AD) i stwardnienia rozsianego (MS).

Nasze wyniki pokazują, że autoimmunomiczną sygnaturę starzenia charakteryzuje falisty wzrost autoprzeciwciał IgG związanych z uczeniem się i zachowaniem oraz stały wzrost autoprzeciwciał IgG związanych z rybosomami i translacją, a autoimmunomiczna sygnatura starzenia jest również związana z neurodegeneracyjną chorobą związaną z wiekiem. choroby. Co ciekawe, Analiza Różnicowej Ekspresji-Sliding Window Evaluation (DE-SWAN) zidentyfikowała trzy fale zmian autoprzeciwciał podczas starzenia się odpowiednio w wieku 30, 50 i 62 lat. Ponadto autoprzeciwciała IgG, w szczególnościte skierowane przeciwko białkom rybosomalnym mogą zostać wykorzystane jako markery prognostyczne dotyczące starzenia się i związanych z wiekiem chorób neurodegeneracyjnych. Dlatego w tym badaniu po raz pierwszy odkryto kompleksowe sygnatury autoimmunologiczne dotyczące starzenia się i związanych z wiekiem chorób neurodegeneracyjnych.

Lenti ORF clone of Nudcd3 (Myc-DDK-tagged) - Mouse NudC domain containing 3 (Nudcd3)
MR205579L3	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Nudcd3 (mGFP-tagged ORF) - Rat NudC domain containing 3 (Nudcd3), (10 ug)
RR208440L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Nudcd3 (Myc-DDK-tagged ORF) - Rat NudC domain containing 3 (Nudcd3), (10 ug)
RR208440L3	Origene Technologies GmbH	10 µg	Ask for price

NUDCD3 cloning plasmid
CSB-CL811605HU1-10ug	Cusabio	10ug	EUR 279.6
Description: A cloning plasmid for the NUDCD3 gene.

NUDCD3 cloning plasmid
CSB-CL811605HU2-10ug	Cusabio	10ug	EUR 495.6
Description: A cloning plasmid for the NUDCD3 gene.

NUDCD3
CSB-CL811605HU1	Cusabio	10 μg plasmid + 200μl Glycerol	Ask for price

NUDCD3
CSB-CL811605HU2	Cusabio	10 μg plasmid + 200μl Glycerol	Ask for price

NUDCD3
E8ER1914-23	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

NUDCD3
MBS8578109-01mL	MyBiosource	0.1mL	EUR 345

NUDCD3
MBS8578109-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

NUDCD3
MBS8578109-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

NUDCD3
MBS8578109-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

NUDCD3
MBS8578109-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

NUDCD3
MBS8564047-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

NUDCD3
MBS8564047-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

NUDCD3
MBS8564047-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

NUDCD3
MBS8564047-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

NUDCD3
MBS8564047-02mL	MyBiosource	0.2mL	EUR 345

Lenti ORF clone of Human NudC domain containing 3 (NUDCD3), mGFP tagged
RC206181L2	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Human NudC domain containing 3 (NUDCD3), mGFP tagged
RC206181L4	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Human NudC domain containing 3 (NUDCD3), Myc-DDK-tagged
RC206181L1	Origene Technologies GmbH	10 µg	Ask for price

Lenti ORF clone of Human NudC domain containing 3 (NUDCD3), Myc-DDK-tagged
RC206181L3	Origene Technologies GmbH	10 µg	Ask for price

NUDCD3 (untagged)-Human NudC domain containing 3 (NUDCD3)
SC112643	Origene Technologies GmbH	10 µg	Ask for price

NUDCD3 (untagged)-Human NudC domain containing 3 (NUDCD3)
SC317579	Origene Technologies GmbH	10 µg	Ask for price

NUDCD3 antibody
70R-3285	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal NUDCD3 antibody raised against the middle region of NUDCD3

NUDCD3 Antibody
42980	SAB	100ul	EUR 319

NUDCD3 Antibody
42980-100ul	SAB	100ul	EUR 302.4

NUDCD3 Antibody
1-CSB-PA811605LA01HU	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

NUDCD3 Antibody
GWB-MT748A	GenWay Biotech	50ug	Ask for price

NUDCD3 Antibody
E042980	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

NUDCD3 Antibody
E312158	EnoGene	200ul	EUR 275
Description: Available in various conjugation types.

NUDCD3 Antibody
MBS1493027-005mg	MyBiosource	0.05mg	EUR 190

NUDCD3 Antibody
MBS1493027-01mg	MyBiosource	0.1mg	EUR 270

NUDCD3 Antibody
MBS1493027-5x01mg	MyBiosource	5x0.1mg	EUR 1205

NUDCD3 antibody
MBS839669-01mL	MyBiosource	0.1mL	EUR 750

NUDCD3 antibody
MBS839669-5x01mL	MyBiosource	5x0.1mL	EUR 3215

NUDCD3 Antibody
MBS9415927-01mL	MyBiosource	0.1mL	EUR 305

NUDCD3 Antibody
MBS9415927-5x01mL	MyBiosource	5x0.1mL	EUR 1230

pENTR223- NUDCD3
PVT11465	Lifescience Market	2 ug	EUR 327.6

Nudcd3 (GFP-tagged) - Mouse NudC domain containing 3 (Nudcd3)
MG205579	Origene Technologies GmbH	10 µg	Ask for price

NUDCD3 (GFP-tagged) - Human NudC domain containing 3 (NUDCD3)
RG206181	Origene Technologies GmbH	10 µg	Ask for price

NUDCD3 Rabbit pAb
A18436	Abclonal	50μL	EUR 70.85

NUDCD3 Rabbit pAb
A18436-100ul	Abclonal	100 ul	EUR 369.6

NUDCD3 Rabbit pAb
A18436-200ul	Abclonal	200 ul	EUR 550.8

NUDCD3 Rabbit pAb
A18436-20ul	Abclonal	20 ul	EUR 219.6

NUDCD3 Rabbit pAb
A18436-50ul	Abclonal	50 ul	EUR 267.6

NUDCD3 Rabbit pAb
E2823895	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

NUDCD3 Rabbit pAb
MBS9143332-002mL	MyBiosource	0.02mL	EUR 200

NUDCD3 Rabbit pAb
MBS9143332-005mL	MyBiosource	0.05mL	EUR 255

NUDCD3 Rabbit pAb
MBS9143332-01mL	MyBiosource	0.1mL	EUR 345

NUDCD3 Rabbit pAb
MBS9143332-02mL	MyBiosource	0.2mL	EUR 545

NUDCD3 Rabbit pAb
MBS9143332-5x02mL	MyBiosource	5x0.2mL	EUR 2265

NUDCD3 Rabbit pAb
MBS8559724-01mL	MyBiosource	0.1mL	EUR 305

NUDCD3 Rabbit pAb
MBS8559724-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

NUDCD3 Rabbit pAb
MBS8559724-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

NUDCD3 Rabbit pAb
MBS8559724-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

NUDCD3 Rabbit pAb
MBS8559724-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

Mouse NUDCD3 siRNA
20-abx926554	Abbexa	Ask for price Ask for price	15 nmol 30 nmol

Human NUDCD3 siRNA
20-abx926555	Abbexa	Ask for price Ask for price	15 nmol 30 nmol

NUDCD3 siRNA (Human)
MBS8230856-15nmol	MyBiosource	15nmol	EUR 405

NUDCD3 siRNA (Human)
MBS8230856-30nmol	MyBiosource	30nmol	EUR 565

NUDCD3 siRNA (Human)
MBS8230856-5x30nmol	MyBiosource	5x30nmol	EUR 2450

NUDCD3 siRNA (Mouse)
MBS8232849-15nmol	MyBiosource	15nmol	EUR 405

NUDCD3 siRNA (Mouse)
MBS8232849-30nmol	MyBiosource	30nmol	EUR 565

NUDCD3 siRNA (Mouse)
MBS8232849-5x30nmol	MyBiosource	5x30nmol	EUR 2450

Nudcd3 (Myc-DDK-tagged) - Mouse NudC domain containing 3 (Nudcd3)
MR205579	Origene Technologies GmbH	10 µg	Ask for price

NUDCD3 (Myc-DDK-tagged)-Human NudC domain containing 3 (NUDCD3)
RC206181	Origene Technologies GmbH	10 µg	Ask for price

anti- NUDCD3 antibody
FNab05897	FN Test	100µg	EUR 702
Description: Antibody raised against NUDCD3

Nudcd3 (untagged ORF) - Rat NudC domain containing 3 (Nudcd3), (10 ug)
RN208440	Origene Technologies GmbH	10 µg	Ask for price

NUDCD3 Blocking Peptide
33R-4441	Fitzgerald	100 ug	EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of NUDCD3 antibody, catalog no. 70R-3285

NUDCD3 Polyclonal Antibody
A60090	EpiGentek	Ask for price Ask for price Ask for price Ask for price	100 µg 20 ul 50 ul 100 ul

NUDCD3 Conjugated Antibody
C42980	SAB	100ul	EUR 476.4

NUDCD3 Polyclonal Antibody
MBS2560307-002mL	MyBiosource	0.02mL	EUR 140

NUDCD3 Polyclonal Antibody
MBS2560307-006mL	MyBiosource	0.06mL	EUR 180

NUDCD3 Polyclonal Antibody
MBS2560307-012mL	MyBiosource	0.12mL	EUR 260

NUDCD3 Polyclonal Antibody
MBS2560307-02mL	MyBiosource	0.2mL	EUR 405

NUDCD3 Polyclonal Antibody
MBS2560307-5x02mL	MyBiosource	5x0.2mL	EUR 1725

NUDCD3 Polyclonal Antibody
MBS9234851-01mL	MyBiosource	0.1mL	EUR 415

NUDCD3 Polyclonal Antibody
MBS9234851-5x01mL	MyBiosource	5x0.1mL	EUR 1841

NUDCD3 Conjugated Antibody
MBS9451312-01mLAF350	MyBiosource	0.1mL(AF350)	EUR 480

NUDCD3 Conjugated Antibody
MBS9451312-01mLAF405	MyBiosource	0.1mL(AF405)	EUR 480

NUDCD3 Conjugated Antibody
MBS9451312-01mLAF488	MyBiosource	0.1mL(AF488)	EUR 480

NUDCD3 Conjugated Antibody
MBS9451312-01mLAF555	MyBiosource	0.1mL(AF555)	EUR 480

NUDCD3 Conjugated Antibody
MBS9451312-01mLBiotin	MyBiosource	0.1mL(Biotin)	EUR 480

NUDCD3 ELISA KIT|Human
EF001364	Lifescience Market	96 Tests	EUR 826.8

Nudcd3 (Myc-DDK-tagged ORF) - Rat NudC domain containing 3 (Nudcd3), (10 ug)
RR208440	Origene Technologies GmbH	10 µg	Ask for price

Mouse NUDCD3 shRNA Plasmid
20-abx980627	Abbexa	Ask for price Ask for price	150 µg 300 µg

Human NUDCD3 shRNA Plasmid
20-abx958230	Abbexa	Ask for price Ask for price	150 µg 300 µg

Human NUDCD3 Protein Lysate
MBS8416325-002mg	MyBiosource	0.02mg	EUR 365

Human NUDCD3 Protein Lysate
MBS8416325-5x002mg	MyBiosource	5x0.02mg	EUR 1410

NUDCD3 Peptide - middle region
MBS3237058-01mg	MyBiosource	0.1mg	EUR 180

NUDCD3 Peptide - middle region
MBS3237058-5x01mg	MyBiosource	5x0.1mg	EUR 730

NUDCD3 Antibody - middle region
MBS3219331-01mL	MyBiosource	0.1mL	EUR 455

NUDCD3 Antibody - middle region
MBS3219331-5x01mL	MyBiosource	5x0.1mL	EUR 1995

NUDCD3 antibody - middle region
MBS3212111-01mL	MyBiosource	0.1mL	EUR 455

NUDCD3 antibody - middle region
MBS3212111-5x01mL	MyBiosource	5x0.1mL	EUR 1995

NUDCD3 Antibody, HRP conjugated
1-CSB-PA811605LB01HU	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

NUDCD3 Antibody, HRP conjugated
MBS1494584-005mg	MyBiosource	0.05mg	EUR 190

NUDCD3 Antibody, HRP conjugated
MBS1494584-01mg	MyBiosource	0.1mg	EUR 270

NUDCD3 Antibody, HRP conjugated
MBS1494584-5x01mg	MyBiosource	5x0.1mg	EUR 1205

NUDCD3 Recombinant Protein (Rat)
RP214763	ABM	100 ug	Ask for price

NUDCD3 Antibody, FITC conjugated
1-CSB-PA811605LC01HU	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

NUDCD3 Antibody, FITC conjugated
MBS1495855-005mg	MyBiosource	0.05mg	EUR 190

NUDCD3 Antibody, FITC conjugated
MBS1495855-01mg	MyBiosource	0.1mg	EUR 270

NUDCD3 Antibody, FITC conjugated
MBS1495855-5x01mg	MyBiosource	5x0.1mg	EUR 1205

Recombinant Human NUDCD3 Protein
E30P64669A	EnoGene	50 ug	EUR 295

NUDCD3 Recombinant Protein (Human)
RP021817	ABM	100 ug	Ask for price

NUDCD3 Recombinant Protein (Human)
RP021820	ABM	100 ug	Ask for price

NUDCD3 Recombinant Protein (Mouse)
RP155360	ABM	100 ug	Ask for price

NUDCD3 Antibody, Biotin conjugated
1-CSB-PA811605LD01HU	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

NUDCD3 Rabbit Polyclonal Antibody
E10G02195	EnoGene	100 μl	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

NUDCD3 Rabbit Polyclonal Antibody
E10G09167	EnoGene	100 μl	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

NUDCD3 Antibody, Biotin conjugated
MBS1495746-005mg	MyBiosource	0.05mg	EUR 190

NUDCD3 Antibody, Biotin conjugated
MBS1495746-01mg	MyBiosource	0.1mg	EUR 270

NUDCD3 Antibody, Biotin conjugated
MBS1495746-5x01mg	MyBiosource	5x0.1mg	EUR 1205

Nudcd3 ORF Vector (Rat) (pORF)
ORF071589	ABM	1.0 ug DNA	EUR 607.2

Nudcd3 ORF Vector (Mouse) (pORF)
ORF051788	ABM	1.0 ug DNA	EUR 607.2

NUDCD3 ORF Vector (Human) (pORF)
ORF007273	ABM	1.0 ug DNA	EUR 114

NUDCD3 ORF Vector (Human) (pORF)
ORF007274	ABM	1.0 ug DNA	EUR 114

NUDCD3 (NM_015332) Human Over-expression Lysate
E45H09601-2	EnoGene	each	EUR 395

NUDCD3 (NM_015332) Human Over-expression Lysate
LS023386	BosterBio	100ug	EUR 628
Description: Transient overexpression lysate of NudC domain containing 3 (NUDCD3)

Human NUDCD3 Protein Lysate 20ug
IHUNUDCD3PLLY20UG	Innovative research	each	EUR 213
Description: Human NUDCD3 Protein Lysate 20ug

Lenti ORF particles, Nudcd3 (GFP-tagged) - Mouse NudC domain containing 3 (Nudcd3), 200ul, >10^7 TU/mL
MR205579L4V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, NUDCD3 (mGFP-tagged) - Human NudC domain containing 3 (NUDCD3), 200ul, >10^7 TU/mL
RC206181L2V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, NUDCD3 (mGFP-tagged) - Human NudC domain containing 3 (NUDCD3), 200ul, >10^7 TU/mL
RC206181L4V	Origene Technologies GmbH	200 µl	Ask for price

OCOA10708-20UG - NUDCD3 Protein Lysate
OCOA10708-20UG	Aviva Systems Biology	20ug	EUR 169

Lenti ORF particles, Nudcd3 (GFP-tagged ORF) - Rat NudC domain containing 3 (Nudcd3), 200ul, >10^7 TU/mL
RR208440L4V	Origene Technologies GmbH	200 µl	Ask for price

NUDCD3 Over-expression Lysate Product
GWB-91F842	GenWay Biotech	0.1 mg	Ask for price

Lenti ORF particles, NUDCD3 (Myc-DDK tagged) - Human NudC domain containing 3 (NUDCD3), 200ul, >10^7 TU/mL
RC206181L1V	Origene Technologies GmbH	200 µl	Ask for price

Lenti ORF particles, NUDCD3 (Myc-DDK tagged) - Human NudC domain containing 3 (NUDCD3), 200ul, >10^7 TU/mL
RC206181L3V	Origene Technologies GmbH	200 µl	Ask for price