jednocząsteczkowego FRET do badania kinetyki   zmian konformacyjnych białek

Połączenie szybkiego mieszania mikroprzepływowego i trójkolorowego jednocząsteczkowego FRET do badania kinetyki   zmian konformacyjnych białek

Jednocząsteczkowy rezonansowy switch energii Förstera (FRET) doskonale nadaje się do badania kinetyki zmian konformacyjnych białek ze względu na wysoką czułość i zdolność do rozpoznawania poszczególnych subpopulacji w układach heterogenicznych. Jednak najpowszechniejsze podejście wykorzystujące dwa fluorofory może monitorować tylko jedną odległość na raz, a użycie trzech fluoroforów do jednoczesnego monitorowania wielu odległości zostało w dużej mierze ograniczone do fluktuacji równowagi. Tutaj pokazujemy, że trójkolorowy jednocząsteczkowy FRET można łączyć z szybkim mieszaniem mikroprzepływowym w celu zbadania zmian konformacyjnych w białku od milisekund do minut. W połączeniu z mieszaniem ręcznym wydłużyliśmy kinetykę do 1 godziny, co odpowiada łącznemu zakresowi 5 rzędów wielkości w czasie.

Zbadaliśmy konwersję monomeru w protomer tworzącej pory toksyny, cytolizyny A (ClyA), jedną z największych znanych przemian konformacyjnych białka. Specyficzne dla miejsca znakowanie ClyA trzema fluoroforami umożliwiło nam jednoczesne śledzenie kinetyki trzech odległości wewnątrzcząsteczkowych i ujawniło wcześniej niewykryty związek pośredni. Połączenie trójkolorowego jednocząsteczkowego FRET z szybkim mieszaniem mikroprzepływowym zapewnia zatem podejście do badania mechanizmów złożonych procesów biomolekularnych z wysoką rozdzielczością czasową.

 

Identyfikacja 7 000-9 000  białek  z linii komórkowych i tkanek za pomocą mikroprzepływu pojedynczego strzału LC-MS/MS

Obecnym trendem w proteomice jest akwizycja danych w „pojedynczym ujęciu” za pomocą LC-MS/MS, ponieważ upraszcza to przepływy pracy i obiecuje lepszą przepustowość i dokładność ilościową niż schematy obejmujące rozległe frakcjonowanie próbek. Jednak podejścia jednokrotne mogą ucierpieć z powodu ograniczonego pokrycia proteomu, gdy są wykonywane przez akwizycję zależną od danych (ssDDA) w systemach nanoprzepływowych LC. W przypadku zastosowań, w których ilości próbek nie są ograniczone, badanie to pokazuje, że wysokie pokrycie proteomem można uzyskać przy użyciu systemu mikroprzepływowego LC-MS/MS obsługującego kolumnę o średnicy wewnętrznej 1 mm × 150 mm, przy natężeniu przepływu 50 μl/min i sprzężonych do spektrometru masowego Orbitrap HF-X.

Wyniki wskazują na identyfikację ~9000 białek z 50 μg strawionego białka z   korzeni Arabidopsis , 7500 z mysiej grasicy i 7300 z ludzkich komórek raka piersi w ciągu three godzin analizy w jednym przebiegu. Dynamiczny zakres oznaczania ilościowego białek mierzony metodą iBAQ obejmował 5 rzędów wielkości, a analiza powtórzeń wykazała, że ​​mediana współczynnika zmienności wynosiła poniżej 20%. Podsumowując , badanie to pokazuje, że ssDDA metodą μLC-MS/MS jest solidną metodą kompleksowej i wielkoskalowej analizy proteomu, którą w przyszłości można rozszerzyć o szybszą chromatografię i metody pozyskiwania danych niezależnych od danych.̀.

miroslawstraczynski
miroslawstraczynski

WDR41 cDNA Clone

MBS1274442-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR91 cDNA Clone

MBS1274578-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR91 cDNA Clone

MBS1274578-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR34 cDNA Clone

MBS1274709-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR34 cDNA Clone

MBS1274709-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR85 cDNA Clone

MBS1274748-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR85 cDNA Clone

MBS1274748-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR13 cDNA Clone

MBS1275052-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR13 cDNA Clone

MBS1275052-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR65 cDNA Clone

MBS1275851-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 435

WDR65 cDNA Clone

MBS1275851-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1910

WDR53 cDNA Clone

MBS1276140-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR53 cDNA Clone

MBS1276140-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR90 cDNA Clone

MBS1276163-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 315

WDR90 cDNA Clone

MBS1276163-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1385

WDR73 cDNA Clone

MBS1277186-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 385

WDR73 cDNA Clone

MBS1277186-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1690

WDR76 cDNA Clone

MBS1277238-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR76 cDNA Clone

MBS1277238-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR19 cDNA Clone

MBS1277262-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR19 cDNA Clone

MBS1277262-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR85 cDNA Clone

MBS1277295-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR85 cDNA Clone

MBS1277295-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR67 cDNA Clone

MBS1277315-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR67 cDNA Clone

MBS1277315-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR75 cDNA Clone

MBS1277561-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 780

WDR75 cDNA Clone

MBS1277561-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3455

WDR20 cDNA Clone

MBS1277815-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR20 cDNA Clone

MBS1277815-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR89 cDNA Clone

MBS1277972-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR89 cDNA Clone

MBS1277972-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR61 cDNA Clone

MBS1278250-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR61 cDNA Clone

MBS1278250-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR62 cDNA Clone

MBS1278452-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR62 cDNA Clone

MBS1278452-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR59 cDNA Clone

MBS1267248-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR59 cDNA Clone

MBS1267248-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR77 cDNA Clone

MBS1267348-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR77 cDNA Clone

MBS1267348-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR76 cDNA Clone

MBS1267434-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR76 cDNA Clone

MBS1267434-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR44 cDNA Clone

MBS1267496-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 600

WDR44 cDNA Clone

MBS1267496-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2655

WDR88 cDNA Clone

MBS1268193-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR88 cDNA Clone

MBS1268193-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR47 cDNA Clone

MBS1268397-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 520

WDR47 cDNA Clone

MBS1268397-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2295

WDR54 cDNA Clone

MBS1268926-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 175

WDR54 cDNA Clone

MBS1268926-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 735

WDR31 cDNA Clone

MBS1269060-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR31 cDNA Clone

MBS1269060-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR82 cDNA Clone

MBS1269111-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR82 cDNA Clone

MBS1269111-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR86 cDNA Clone

MBS1269205-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 275

WDR86 cDNA Clone

MBS1269205-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1190

WDR33 cDNA Clone

MBS1269354-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR33 cDNA Clone

MBS1269354-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR92 cDNA Clone

MBS1269465-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR92 cDNA Clone

MBS1269465-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR25 cDNA Clone

MBS1269628-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR25 cDNA Clone

MBS1269628-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR34 cDNA Clone

MBS1269761-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 425

WDR34 cDNA Clone

MBS1269761-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1860

WDR38 cDNA Clone

MBS1269862-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR38 cDNA Clone

MBS1269862-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR27 cDNA Clone

MBS1269863-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 435

WDR27 cDNA Clone

MBS1269863-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1910

WDR20 cDNA Clone

MBS1271465-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR20 cDNA Clone

MBS1271465-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR63 cDNA Clone

MBS1271597-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 520

WDR63 cDNA Clone

MBS1271597-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2295

WDR37 cDNA Clone

MBS1271703-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR37 cDNA Clone

MBS1271703-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR74 cDNA Clone

MBS1271879-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR74 cDNA Clone

MBS1271879-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR18 cDNA Clone

MBS1271880-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 435

WDR18 cDNA Clone

MBS1271880-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1910

WDR60 cDNA Clone

MBS1272194-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1155

WDR60 cDNA Clone

MBS1272194-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 5150

WDR66 cDNA Clone

MBS1273393-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 880

WDR66 cDNA Clone

MBS1273393-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3910

WDR47 cDNA Clone

MBS1273453-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 520

WDR47 cDNA Clone

MBS1273453-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2295

WDR54 cDNA Clone

MBS1273667-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR54 cDNA Clone

MBS1273667-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR24 cDNA Clone

MBS1273900-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 745

WDR24 cDNA Clone

MBS1273900-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3300

WDR16 cDNA Clone

MBS1266694-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR16 cDNA Clone

MBS1266694-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR66 cDNA Clone

MBS1266972-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1245

WDR66 cDNA Clone

MBS1266972-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 5560

WDR49 cDNA Clone

MBS1270547-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 435

WDR49 cDNA Clone

MBS1270547-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1910

WDR18 cDNA Clone

MBS1270831-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR18 cDNA Clone

MBS1270831-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR41 cDNA Clone

MBS1278785-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 350

WDR41 cDNA Clone

MBS1278785-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1525

Lenti ORF clone of Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514)

MR204616L3 10 µg Ask for price

WDR45L cDNA Clone

MBS1268101-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR45L cDNA Clone

MBS1268101-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR5 cDNA Clone

MBS1275747-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR5 cDNA Clone

MBS1275747-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR4 cDNA Clone

MBS1276184-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR4 cDNA Clone

MBS1276184-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR1 cDNA Clone

MBS1267204-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR1 cDNA Clone

MBS1267204-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR6 cDNA Clone

MBS1267661-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1210

WDR6 cDNA Clone

MBS1267661-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 5405

WDR6 cDNA Clone

MBS1268803-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR6 cDNA Clone

MBS1268803-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR4 cDNA Clone

MBS1269790-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR4 cDNA Clone

MBS1269790-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR3 cDNA Clone

MBS1272968-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 160

WDR3 cDNA Clone

MBS1272968-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 670

WDR1 cDNA Clone

MBS1273887-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR1 cDNA Clone

MBS1273887-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR7 cDNA Clone

MBS1274012-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 765

WDR7 cDNA Clone

MBS1274012-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3400

Lenti ORF particles, Wdr78 (GFP-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514), 200ul, >10^7 TU/mL

MR204616L4V 200 µl Ask for price

Lenti ORF particles, Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514), 200ul, >10^7 TU/mL

MR204616L3V 200 µl Ask for price

Lenti ORF clone of Wdr78 (mGFP-tagged) - Mouse WD repeat domain 78 (Wdr78)

MR209396L4 10 µg Ask for price

Lenti ORF clone of Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (Wdr78)

MR209396L3 10 µg Ask for price

Lenti ORF clone of Wdr78 (mGFP-tagged ORF) - Rat WD repeat domain 78 (Wdr78), (10 ug)

RR208219L4 10 µg Ask for price

Lenti ORF clone of Wdr78 (Myc-DDK-tagged ORF) - Rat WD repeat domain 78 (Wdr78), (10 ug)

RR208219L3 10 µg Ask for price

Lenti-ORF clone of WDR78 (mGFP-tagged)-Human WD repeat domain 78 (WDR78), transcript variant 2

RC221613L4 10 µg Ask for price

Lenti ORF clone of Wdr48 (mGFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)

MR209851L4 10 µg Ask for price

Lenti ORF clone of Wdr62 (mGFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)

MR211422L4 10 µg Ask for price

Lenti ORF clone of Wdr62 (mGFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)

MR211595L4 10 µg Ask for price

Lenti ORF clone of Wdr73 (mGFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)

MR203438L4 10 µg Ask for price

Lenti ORF clone of Wdr20a (mGFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)

MR208616L4 10 µg Ask for price

Lenti ORF clone of Wdr42a (mGFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)

MR204846L4 10 µg Ask for price

Lenti ORF clone of Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)

MR209851L3 10 µg Ask for price

Lenti ORF clone of Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)

MR211422L3 10 µg Ask for price

Lenti ORF clone of Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)

MR211595L3 10 µg Ask for price

Lenti ORF clone of Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)

MR203438L3 10 µg Ask for price

Lenti-ORF clone of WDR78 (Myc-DDK-tagged)-Human WD repeat domain 78 (WDR78), transcript variant 2

RC221613L3 10 µg Ask for price

Lenti ORF clone of Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)

MR208616L3 10 µg Ask for price

Lenti ORF clone of Wdr42a (Myc-DDK-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)

MR204846L3 10 µg Ask for price

WDR3 (untagged)-Human cDNA clone MGC:61946 IMAGE:6745281, complete cds

SC126422 10 µg Ask for price

Wdr48 (GFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)

MG209851 10 µg Ask for price

Wdr36 (GFP-tagged) - Mouse WD repeat domain 36 (cDNA clone MGC:103340 IMAGE:5068941)

MG211095 10 µg Ask for price

Wdr47 (GFP-tagged) - Mouse WD repeat domain 47 (cDNA clone MGC:8037 IMAGE:3586908)

MG211187 10 µg Ask for price

Wdr59 (GFP-tagged) - Mouse WD repeat domain 59 (cDNA clone MGC:91308 IMAGE:30355918)

MG211357 10 µg Ask for price

Wdr67 (GFP-tagged) - Mouse WD repeat domain 67 (cDNA clone MGC:60990 IMAGE:30016266)

MG211408 10 µg Ask for price

Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)

MG211422 10 µg Ask for price

Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)

MG211595 10 µg Ask for price

Wdr32 (GFP-tagged) - Mouse WD repeat domain 32 (cDNA clone MGC:11993 IMAGE:3601889)

MG206473 10 µg Ask for price

Wdr73 (GFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)

MG203438 10 µg Ask for price

Wdr77 (untagged) - Mouse WD repeat domain 77 (cDNA clone MGC:6890 IMAGE:2654151), (10ug)

MC200277 10 µg Ask for price

Wdr62 (untagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), (10ug)

MC223031 10 µg Ask for price

Wdr62 (untagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), (10ug)

MC223128 10 µg Ask for price

Wdr48 (untagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), (10ug)

MC217839 10 µg Ask for price

Wdr73 (untagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), (10ug)

MC218435 10 µg Ask for price

Wdr37 (untagged) - Mouse WD repeat domain 37 (cDNA clone MGC:58091 IMAGE:6817089), (10ug)

MC206152 10 µg Ask for price

Wdr24 (untagged) - Mouse WD repeat domain 24 (cDNA clone MGC:47001 IMAGE:5148210), (10ug)

MC206644 10 µg Ask for price

Wdr68 (untagged) - Mouse WD repeat domain 68 (cDNA clone MGC:58053 IMAGE:6409531), (10ug)

MC206690 10 µg Ask for price

Wdr20a (GFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)

MG208616 10 µg Ask for price

Wdr42a (GFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)

MG204846 10 µg Ask for price

Wdr20a (untagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717), (10ug)

MC217866 10 µg Ask for price

Wdr42a (untagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38511 IMAGE:5352941), (10ug)

MC206626 10 µg Ask for price

Wdr40b (untagged) - Mouse WD repeat domain 40B (cDNA clone MGC:76797 IMAGE:30431243), (10ug)

MC206734 10 µg Ask for price

WDR78 cloning plasmid

CSB-CL719620HU1-10ug 10ug
EUR 640.8
Description: A cloning plasmid for the WDR78 gene.

WDR78 cloning plasmid

CSB-CL719620HU2-10ug 10ug
EUR 986.4
Description: A cloning plasmid for the WDR78 gene.

Lenti ORF clone of Wdr4 (mGFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)

MR206503L4 10 µg Ask for price

Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)

MR209851 10 µg Ask for price

Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)

MR211422 10 µg Ask for price

Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)

MR211595 10 µg Ask for price

Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)

MR203438 10 µg Ask for price

Lenti ORF clone of Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)

MR206503L3 10 µg Ask for price

Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)

MR208616 10 µg Ask for price

Wdr42a (Myc-DDK-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)

MR204846 10 µg Ask for price

WDR37 (untagged)-Human WD repeat domain 37 (cDNA clone MGC:26330 IMAGE:4794184), complete cds

SC123593 10 µg Ask for price

Wdr4 (GFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)

MG206503 10 µg Ask for price

Wdr8 (untagged) - Mouse WD repeat domain 8 (cDNA clone MGC:28791 IMAGE:4501325), (10ug)

MC218347 10 µg Ask for price

WDR59 (untagged)-Human hypothetical protein FLJ12270 (cDNA clone MGC:11230 IMAGE:3938206), complete cds

SC122523 10 µg Ask for price

WDR77 (untagged)-Human methylosome protein 50, mRNA (cDNA clone MGC:4346 IMAGE:2822173), complete cds

SC126139 10 µg Ask for price

Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)

MR206503 10 µg Ask for price

WDR78

CSB-CL719620HU1 10 μg plasmid + 200μl Glycerol Ask for price

WDR78

CSB-CL719620HU2 10 μg plasmid + 200μl Glycerol Ask for price

WDR25 (untagged)-Human pre-mRNA splicing factor-like (cDNA clone MGC:4645 IMAGE:3529568), complete cds

SC122376 10 µg Ask for price

WDR85 (untagged)-Human chromosome 9 open reading frame 112, mRNA (cDNA clone MGC:3209 IMAGE:3502107), complete cds

SC126100 10 µg Ask for price

Lenti ORF particles, Wdr48 (GFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), 200ul, >10^7 TU/mL

MR209851L4V 200 µl Ask for price

Lenti ORF particles, Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), 200ul, >10^7 TU/mL

MR211422L4V 200 µl Ask for price

Lenti ORF particles, Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), 200ul, >10^7 TU/mL

MR211595L4V 200 µl Ask for price

Lenti ORF particles, Wdr73 (GFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), 200ul, >10^7 TU/mL

MR203438L4V 200 µl Ask for price

Lenti ORF particles, Wdr20a (GFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717), 200ul, >10^7 TU/mL

MR208616L4V 200 µl Ask for price

Lenti ORF particles, Wdr42a (GFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701), 200ul, >10^7 TU/mL

MR204846L4V 200 µl Ask for price

Lenti ORF particles, Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), 200ul, >10^7 TU/mL

MR209851L3V 200 µl Ask for price

Lenti ORF particles, Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), 200ul, >10^7 TU/mL

MR211422L3V 200 µl Ask for price

Lenti ORF particles, Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), 200ul, >10^7 TU/mL

MR211595L3V 200 µl Ask for price

Lenti ORF particles, Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), 200ul, >10^7 TU/mL

MR203438L3V 200 µl Ask for price

WDR78 siRNA

20-abx906072
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

WDR78 siRNA

20-abx939775
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

WDR78 siRNA

20-abx939776
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

Lenti ORF particles, Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717), 200ul, >10^7 TU/mL

MR208616L3V 200 µl Ask for price

Lenti ORF particles, Wdr42a (Myc-DDK-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701), 200ul, >10^7 TU/mL

MR204846L3V 200 µl Ask for price

Immunoterapia raka z użyciem NC410, białka LAIR-2 Fc   blokującego interakcję ludzkiego LAIR-kolagen 

Kolageny są głównym składnikiem macierzy zewnątrzkomórkowej i są funkcjonalnymi ligandami dla hamującego receptora immunologicznego związanego z leukocytami receptora podobnego do immunoglobuliny (LAIR)-1. LAIR-2 to wydzielane białko, które może działać jako receptor wabikowy, wiążąc kolagen z wyższym powinowactwem niż LAIR-1. Proponujemy, że kolageny promują unikanie odporności poprzez interakcję z LAIR-1 eksprymowanym na komórkach odpornościowych i że LAIR-2 uwalnia supresję immunologiczną za pośrednictwem LAIR-1.

Analiza publicznych zbiorów danych dotyczących ludzi pokazuje, że kolageny, LAIR-1 i LAIR-2 mają unikalne i nakładające się związki z przeżyciem w niektórych nowotworach. Zaprojektowaliśmy dimeryczny LAIR-2 z funkcjonalnym ogonem IgG1 Fc, NC410, i wykazaliśmy, że NC410 zwiększa ekspansję ludzkich komórek T i funkcję efektorową  in vivo  w mysim modelu choroby ksenogenicznego przeszczep przeciwko gospodarzowi. W humanizowanych mysich modelach nowotworów NC410 zmniejsza wzrost nowotworu zależny od komórek T. Analiza immunohistochemiczna ludzkich guzów wykazała, że ​​NC410 wiąże się z obszarami bogatymi w kolagen, w których  zlokalizowane są komórki odpornościowe LAIR-1 + . Nasze odkrycia pokazują, że NC410 może być nową strategią immunoterapii nowotworów w przypadku guzów z wykluczeniem immunologicznym.

Identyfikacja immunologiczna i charakterystyka białka  szkieletowego kapsydu  kodowanego przez UL26.5 wirusa Herpes Simplex typu 2

Wirus opryszczki pospolitej typu 2 (HSV2), patogen powodujący zmiany opryszczkowe narządów płciowych, ingeruje w układ odpornościowy gospodarza  poprzez  różne znane i nieznane mechanizmy. Wirus ten był używany do badania składu antygenowego wirusa. Surowicę rekonwalescencyjną od pacjentów zakażonych HSV2 zastosowano do identyfikacji antygenów wirusowych  za pomocą  elektroforezy białek 2-D i immunoblottingu. Surowica rozpoznawała głównie kilka białek szkieletowych kapsydu kodowanych przez gen UL26.5, głównie ICP35 .

Doniesiono, że białko to działa przejściowo w tworzeniu wirusów, ale nie ulega ekspresji w dojrzałych cząsteczkach wirusa. Dalsze badania immunologiczne sugerowały, że to białko wywołuje u myszy odpowiedzi swoistych przeciwciał i cytotoksycznych limfocytów T (CTL), ale te odpowiedzi nie dają klinicznego efektu ochronnego w odpowiedzi na prowokację HSV2 . Dane sugerują, że immunodominacja ICP35 może być wykorzystana do zaprojektowania zintegrowanego antygenu z innymi glikoproteinami wirusowymi.

Autoimmunologiczne sygnatury starzenia się i chorób neurodegeneracyjnych związanych z wiekiem są powiązane z funkcją mózgu i białkami rybosomalnymi

Biologiczne starzenie się to złożony proces charakteryzujący się upośledzeniem funkcji komórek i tkanek, w tym układu odpornościowego. W konsekwencji starzenie się wpływa na ekspresję i rozwój autoprzeciwciał oraz autoreaktywnych limfocytów T, co w ich sumie można uznać za autoimmunom osobnika. W tym badaniu przeanalizowaliśmy, czy zestawy cech autoimmunologicznych są powiązane z określonymi fenotypami, które tworzą sygnatury autoimmunologiczne związane z wiekiem i chorobami neurodegeneracyjnymi . Dane profilu autoprzeciwciał zdrowych osobników i pacjentów z bazy danych GEO wykorzystano do zbadania autoimmunologicznych sygnatur starzenia i trzech chorób neurodegeneracyjnych, w tym choroby Parkinsona (PD), choroby Alzheimera (AD) i stwardnienia rozsianego (MS).

Nasze wyniki pokazują, że autoimmunomiczną sygnaturę starzenia charakteryzuje falisty wzrost autoprzeciwciał IgG związanych z uczeniem się i zachowaniem oraz stały wzrost autoprzeciwciał IgG związanych z rybosomami i translacją, a autoimmunomiczna sygnatura starzenia jest również związana z neurodegeneracyjną chorobą związaną z wiekiem. choroby. Co ciekawe, Analiza Różnicowej Ekspresji-Sliding Window Evaluation (DE-SWAN) zidentyfikowała trzy fale zmian autoprzeciwciał podczas starzenia się odpowiednio w wieku 30, 50 i 62 lat. Ponadto autoprzeciwciała IgG, w szczególnościte skierowane przeciwko białkom rybosomalnym mogą zostać wykorzystane jako markery prognostyczne dotyczące starzenia się i związanych z wiekiem chorób neurodegeneracyjnych. Dlatego w tym badaniu po raz pierwszy odkryto kompleksowe sygnatury autoimmunologiczne dotyczące starzenia się i związanych z wiekiem chorób neurodegeneracyjnych.

Lenti ORF clone of Nudcd3 (Myc-DDK-tagged) - Mouse NudC domain containing 3 (Nudcd3)

MR205579L3 10 µg Ask for price

Lenti ORF clone of Nudcd3 (mGFP-tagged ORF) - Rat NudC domain containing 3 (Nudcd3), (10 ug)

RR208440L4 10 µg Ask for price

Lenti ORF clone of Nudcd3 (Myc-DDK-tagged ORF) - Rat NudC domain containing 3 (Nudcd3), (10 ug)

RR208440L3 10 µg Ask for price

NUDCD3 cloning plasmid

CSB-CL811605HU1-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the NUDCD3 gene.

NUDCD3 cloning plasmid

CSB-CL811605HU2-10ug 10ug
EUR 495.6
Description: A cloning plasmid for the NUDCD3 gene.

NUDCD3

E8ER1914-23 100ul
EUR 275
Description: Available in various conjugation types.

NUDCD3

CSB-CL811605HU1 10 μg plasmid + 200μl Glycerol Ask for price

NUDCD3

CSB-CL811605HU2 10 μg plasmid + 200μl Glycerol Ask for price

NUDCD3

MBS8578109-01mL 0.1mL
EUR 345

NUDCD3

MBS8578109-01mLAF405L 0.1mL(AF405L)
EUR 565

NUDCD3

MBS8578109-01mLAF405S 0.1mL(AF405S)
EUR 565

NUDCD3

MBS8578109-01mLAF610 0.1mL(AF610)
EUR 565

NUDCD3

MBS8578109-01mLAF635 0.1mL(AF635)
EUR 565

NUDCD3

MBS8564047-01mLAF405L 0.1mL(AF405L)
EUR 565

NUDCD3

MBS8564047-01mLAF405S 0.1mL(AF405S)
EUR 565

NUDCD3

MBS8564047-01mLAF610 0.1mL(AF610)
EUR 565

NUDCD3

MBS8564047-01mLAF635 0.1mL(AF635)
EUR 565

NUDCD3

MBS8564047-02mL 0.2mL
EUR 345

Lenti ORF clone of Human NudC domain containing 3 (NUDCD3), mGFP tagged

RC206181L2 10 µg Ask for price

Lenti ORF clone of Human NudC domain containing 3 (NUDCD3), mGFP tagged

RC206181L4 10 µg Ask for price

Lenti ORF clone of Human NudC domain containing 3 (NUDCD3), Myc-DDK-tagged

RC206181L1 10 µg Ask for price

Lenti ORF clone of Human NudC domain containing 3 (NUDCD3), Myc-DDK-tagged

RC206181L3 10 µg Ask for price

NUDCD3 siRNA

20-abx926554
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

NUDCD3 siRNA

20-abx926555
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

NUDCD3 (untagged)-Human NudC domain containing 3 (NUDCD3)

SC112643 10 µg Ask for price

NUDCD3 (untagged)-Human NudC domain containing 3 (NUDCD3)

SC317579 10 µg Ask for price

NUDCD3 Antibody

42980 100ul
EUR 319

NUDCD3 Antibody

42980-100ul 100ul
EUR 302.4

NUDCD3 Antibody

1-CSB-PA811605LA01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

NUDCD3 Antibody

E042980 100μg/100μl
EUR 255
Description: Available in various conjugation types.

NUDCD3 Antibody

E312158 200ul
EUR 275
Description: Available in various conjugation types.

NUDCD3 antibody

70R-3285 50 ug
EUR 467
Description: Rabbit polyclonal NUDCD3 antibody raised against the middle region of NUDCD3

NUDCD3 Antibody

GWB-MT748A 50ug Ask for price

NUDCD3 antibody

MBS839669-01mL 0.1mL
EUR 750

NUDCD3 antibody

MBS839669-5x01mL 5x0.1mL
EUR 3215

NUDCD3 Antibody

MBS1493027-005mg 0.05mg
EUR 190

NUDCD3 Antibody

MBS1493027-01mg 0.1mg
EUR 270

NUDCD3 Antibody

MBS1493027-5x01mg 5x0.1mg
EUR 1205

NUDCD3 Antibody

MBS9415927-01mL 0.1mL
EUR 305

NUDCD3 Antibody

MBS9415927-5x01mL 5x0.1mL
EUR 1230

pENTR223- NUDCD3

PVT11465 2ug
EUR 182

Nudcd3 (GFP-tagged) - Mouse NudC domain containing 3 (Nudcd3)

MG205579 10 µg Ask for price

NUDCD3 (GFP-tagged) - Human NudC domain containing 3 (NUDCD3)

RG206181 10 µg Ask for price

NUDCD3 Rabbit pAb

E2823895 100ul
EUR 225
Description: Available in various conjugation types.

NUDCD3 Rabbit pAb

A18436-100ul 100 ul
EUR 369.6

NUDCD3 Rabbit pAb

A18436-200ul 200 ul
EUR 550.8

NUDCD3 Rabbit pAb

A18436-20ul 20 ul
EUR 219.6

NUDCD3 Rabbit pAb

A18436-50ul 50 ul
EUR 267.6

NUDCD3 Rabbit pAb

MBS9143332-002mL 0.02mL
EUR 200

NUDCD3 Rabbit pAb

MBS9143332-005mL 0.05mL
EUR 255

NUDCD3 Rabbit pAb

MBS9143332-01mL 0.1mL
EUR 345

NUDCD3 Rabbit pAb

MBS9143332-02mL 0.2mL
EUR 545

NUDCD3 Rabbit pAb

MBS9143332-5x02mL 5x0.2mL
EUR 2265

NUDCD3 Rabbit pAb

MBS8559724-01mL 0.1mL
EUR 305

NUDCD3 Rabbit pAb

MBS8559724-01mLAF405L 0.1mL(AF405L)
EUR 565

NUDCD3 Rabbit pAb

MBS8559724-01mLAF405S 0.1mL(AF405S)
EUR 565

NUDCD3 Rabbit pAb

MBS8559724-01mLAF610 0.1mL(AF610)
EUR 565

NUDCD3 Rabbit pAb

MBS8559724-01mLAF635 0.1mL(AF635)
EUR 565

NUDCD3 Rabbit pAb

A18436 50μL
EUR 70.85

Nudcd3 (Myc-DDK-tagged) - Mouse NudC domain containing 3 (Nudcd3)

MR205579 10 µg Ask for price

NUDCD3 (Myc-DDK-tagged)-Human NudC domain containing 3 (NUDCD3)

RC206181 10 µg Ask for price

NUDCD3 siRNA (Mouse)

MBS8232849-15nmol 15nmol
EUR 405

NUDCD3 siRNA (Mouse)

MBS8232849-30nmol 30nmol
EUR 565

NUDCD3 siRNA (Mouse)

MBS8232849-5x30nmol 5x30nmol
EUR 2450

NUDCD3 siRNA (Human)

MBS8230856-15nmol 15nmol
EUR 405

NUDCD3 siRNA (Human)

MBS8230856-30nmol 30nmol
EUR 565

NUDCD3 siRNA (Human)

MBS8230856-5x30nmol 5x30nmol
EUR 2450

anti- NUDCD3 antibody

FNab05897 100µg
EUR 702
Description: Antibody raised against NUDCD3

Nudcd3 (untagged ORF) - Rat NudC domain containing 3 (Nudcd3), (10 ug)

RN208440 10 µg Ask for price

NUDCD3 Blocking Peptide

33R-4441 100 ug
EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of NUDCD3 antibody, catalog no. 70R-3285

NUDCD3 Conjugated Antibody

C42980 100ul
EUR 476.4

NUDCD3 Polyclonal Antibody

A60090
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

NUDCD3 Polyclonal Antibody

MBS9234851-01mL 0.1mL
EUR 415

NUDCD3 Polyclonal Antibody

MBS9234851-5x01mL 5x0.1mL
EUR 1841

NUDCD3 Polyclonal Antibody

MBS2560307-002mL 0.02mL
EUR 140

NUDCD3 Polyclonal Antibody

MBS2560307-006mL 0.06mL
EUR 180

NUDCD3 Polyclonal Antibody

MBS2560307-012mL 0.12mL
EUR 260

NUDCD3 Polyclonal Antibody

MBS2560307-02mL 0.2mL
EUR 405

NUDCD3 Polyclonal Antibody

MBS2560307-5x02mL 5x0.2mL
EUR 1725

NUDCD3 Conjugated Antibody

MBS9451312-01mLAF350 0.1mL(AF350)
EUR 480

NUDCD3 Conjugated Antibody

MBS9451312-01mLAF405 0.1mL(AF405)
EUR 480

NUDCD3 Conjugated Antibody

MBS9451312-01mLAF488 0.1mL(AF488)
EUR 480

NUDCD3 Conjugated Antibody

MBS9451312-01mLAF555 0.1mL(AF555)
EUR 480

NUDCD3 Conjugated Antibody

MBS9451312-01mLBiotin 0.1mL(Biotin)
EUR 480

Human NUDCD3 ELISA KIT

EF001364 96tests
EUR 566

Nudcd3 (Myc-DDK-tagged ORF) - Rat NudC domain containing 3 (Nudcd3), (10 ug)

RR208440 10 µg Ask for price

Mouse NUDCD3 shRNA Plasmid

20-abx980627
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  • 150 µg
  • 300 µg

Human NUDCD3 shRNA Plasmid

20-abx958230
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  • Ask for price
  • 150 µg
  • 300 µg

Human NUDCD3 Protein Lysate

MBS8416325-002mg 0.02mg
EUR 365

Human NUDCD3 Protein Lysate

MBS8416325-5x002mg 5x0.02mg
EUR 1410

NUDCD3 Peptide - middle region

MBS3237058-01mg 0.1mg
EUR 180

NUDCD3 Peptide - middle region

MBS3237058-5x01mg 5x0.1mg
EUR 730

NUDCD3 Antibody - middle region

MBS3219331-01mL 0.1mL
EUR 455

NUDCD3 Antibody - middle region

MBS3219331-5x01mL 5x0.1mL
EUR 1995

NUDCD3 antibody - middle region

MBS3212111-01mL 0.1mL
EUR 455

NUDCD3 antibody - middle region

MBS3212111-5x01mL 5x0.1mL
EUR 1995

NUDCD3 Antibody, HRP conjugated

1-CSB-PA811605LB01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

NUDCD3 Recombinant Protein (Rat)

RP214763 100 ug Ask for price

NUDCD3 Antibody, HRP conjugated

MBS1494584-005mg 0.05mg
EUR 190

NUDCD3 Antibody, HRP conjugated

MBS1494584-01mg 0.1mg
EUR 270

NUDCD3 Antibody, HRP conjugated

MBS1494584-5x01mg 5x0.1mg
EUR 1205

NUDCD3 Antibody, FITC conjugated

1-CSB-PA811605LC01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

NUDCD3 Antibody, FITC conjugated

MBS1495855-005mg 0.05mg
EUR 190

NUDCD3 Antibody, FITC conjugated

MBS1495855-01mg 0.1mg
EUR 270

NUDCD3 Antibody, FITC conjugated

MBS1495855-5x01mg 5x0.1mg
EUR 1205

NUDCD3 Recombinant Protein (Human)

RP021817 100 ug Ask for price

NUDCD3 Recombinant Protein (Human)

RP021820 100 ug Ask for price

NUDCD3 Recombinant Protein (Mouse)

RP155360 100 ug Ask for price

NUDCD3 Antibody, Biotin conjugated

1-CSB-PA811605LD01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

NUDCD3 Rabbit Polyclonal Antibody

E10G09167 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

NUDCD3 Rabbit Polyclonal Antibody

E10G02195 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

NUDCD3 Antibody, Biotin conjugated

MBS1495746-005mg 0.05mg
EUR 190

NUDCD3 Antibody, Biotin conjugated

MBS1495746-01mg 0.1mg
EUR 270

NUDCD3 Antibody, Biotin conjugated

MBS1495746-5x01mg 5x0.1mg
EUR 1205

Nudcd3 ORF Vector (Rat) (pORF)

ORF071589 1.0 ug DNA
EUR 607.2

NUDCD3 ORF Vector (Human) (pORF)

ORF007273 1.0 ug DNA
EUR 114

NUDCD3 ORF Vector (Human) (pORF)

ORF007274 1.0 ug DNA
EUR 114

Nudcd3 ORF Vector (Mouse) (pORF)

ORF051788 1.0 ug DNA
EUR 607.2

NUDCD3 (NM_015332) Human Over-expression Lysate

LS023386 100ug
EUR 628
Description: Transient overexpression lysate of NudC domain containing 3 (NUDCD3)

Human NUDCD3 Protein Lysate 20ug

IHUNUDCD3PLLY20UG each
EUR 213
Description: Human NUDCD3 Protein Lysate 20ug

Lenti ORF particles, Nudcd3 (GFP-tagged) - Mouse NudC domain containing 3 (Nudcd3), 200ul, >10^7 TU/mL

MR205579L4V 200 µl Ask for price

Lenti ORF particles, NUDCD3 (mGFP-tagged) - Human NudC domain containing 3 (NUDCD3), 200ul, >10^7 TU/mL

RC206181L2V 200 µl Ask for price

Lenti ORF particles, NUDCD3 (mGFP-tagged) - Human NudC domain containing 3 (NUDCD3), 200ul, >10^7 TU/mL

RC206181L4V 200 µl Ask for price

OCOA10708-20UG - NUDCD3 Protein Lysate

OCOA10708-20UG 20ug
EUR 169

Lenti ORF particles, Nudcd3 (GFP-tagged ORF) - Rat NudC domain containing 3 (Nudcd3), 200ul, >10^7 TU/mL

RR208440L4V 200 µl Ask for price

NUDCD3 Over-expression Lysate Product

GWB-91F842 0.1 mg Ask for price

Lenti ORF particles, NUDCD3 (Myc-DDK tagged) - Human NudC domain containing 3 (NUDCD3), 200ul, >10^7 TU/mL

RC206181L1V 200 µl Ask for price

Lenti ORF particles, NUDCD3 (Myc-DDK tagged) - Human NudC domain containing 3 (NUDCD3), 200ul, >10^7 TU/mL

RC206181L3V 200 µl Ask for price

Lenti ORF particles, Nudcd3 (Myc-DDK-tagged) - Mouse NudC domain containing 3 (Nudcd3), 200ul, >10^7 TU/mL

MR205579L3V 200 µl Ask for price

NUDCD3 Polyclonal Antibody, HRP Conjugated

A60093
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  • 100 µg
  • 50 ul
  • 100 ul

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